Part:BBa_K2018012:Experience
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2018012
We cloned the part into a purification plasmid (pTXB1) provided from NEB, we then performed a impact purification of 500 ml ON-cuture and ended with 2.5 ml purified protein with a concentration of 44,4 ug/ml.
We then performed a MIC assay to test for antimicrobial effect. The MIC assay where performed on three resistant S. aureus and one P. aeruginosa we got the following results:
| Strain | ug/ml |
| S. aureus:CC398 | 22,2 |
| S. aureus:USA300 | 22,2 |
| S. aureus:hVISA | 22,2 |
| P. aeruginosa:PAO1 | >22,2 |
The idea is that you can use the bacteriocin as a antimicrobial agent how ever it pleases you. We looked into the possibility of coating both the bacteriocin in a compound to make it not digested, or coating stuff in bacteriocins so they may be antimicrobial.
User Reviews
UNIQd8cbec68d26a8fde-partinfo-00000000-QINU UNIQd8cbec68d26a8fde-partinfo-00000001-QINU